Gametocidal chromosomes enhancing chromosome aberration in common wheat induced by 5-azacytidine
نویسندگان
چکیده
منابع مشابه
Gametocidal chromosomes enhancing chromosome aberration in common wheat induced by 5-azacytidine.
The gametocidal (Gc) chromosome from Aegilops spp induces chromosome mutation, which is introduced into common wheat as a tool of chromosome manipulation for genetic improvement. The Gc chromosome functions similar to a restriction-modification system in bacteria, in which DNA methylation is an important regulator. We treated root tips of wheat carrying Gc chromosomes with the hypomethylation a...
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Hypomethylation of DNA, which can be achieved by incorporation of 5-azacytidine, has been correlated with derepression of genes. In order to examine the in vivo effects of 5-azacytidine on organ development and differentiation, young rats were treated with the drug. There was an almost complete reduction of thymus and a marked reduction of spleen weight, while other organs, including testes wer...
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We used two gametocidal (Gc) chromosomes 2C and 3C(SAT) to dissect barley chromosome 4H added to common wheat. The Gc chromosome induced chromosomal structural rearrangements in the progeny of the 4H addition line of common wheat carrying the monosomic Gc chromosome. We conducted in situ hybridization to select plants carrying rearranged 4H chromosomes and characterized the rearranged chromosom...
متن کاملCytological dissection of the triticeae chromosomes by the gametocidal system.
Triticeae species have a large and complex genome, which has made it difficult to obtain their sequence data. Some alien chromosomes called the gametocidal (Gc) chromosomes introduced into common wheat can induce chromosomal breakage resulting in the generation of deletions and translocations. The induced deletions have been established as deletion stocks in common wheat. This Gc system is also...
متن کاملThe Variation Analysis of DNA Methylation in Wheat Carrying Gametocidal Chromosome 3C from Aegilops triuncialis
Gametocidal (Gc) chromosomes can ensure their preferential transmission by killing the gametes without themselves through causing chromosome breakage and therefore have been exploited as an effective tool for genetic breeding. However, to date very little is known about the molecular mechanism of Gc action. In this study, we used methylation-sensitive amplified polymorphism (MSAP) technique to ...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2013
ISSN: 1676-5680
DOI: 10.4238/2013.july.8.4